MOi 01

But here is the tricky part. Adam 20 Januarypm. We use cookies to make MOi 01 with our website easy and meaningful, to better understand the use of our services, and to tailor advertising. Please excuse me in advance that I ask you to explain obvious things. Actually what is a MOi 01 of Poisson distribution. Thank you all for the reply. The suggested incubation MOi 01 stock preparation also around days.

I have studied varicella zoster virus. I had determined the titer of virus that follows your guiderline. But now, I have a trouble with it. I do not know how to find MOI. Can you help me to find out it? Once, thank you very much. You determine the MOI by adding a certain amount of virus to a fixed number of cells. The MOI is the number of infectious viruses added divided by the number of cells. We have an assumption for monolayer cell line that the m. I was talking about a general assumptions with monolayer infection of virus irrespective of the type of virus used for the infection.

How is the Poisson distribution affected when infecting in suspension versus adherent cultures? Obviously there are volumetric effects that would hinder the random associationg of virus bumping into the cells. Thanks for your kind assistance. You have to know the total number of cells in the flask when you infect them. Suspension vs monolayer does not matter in calculating MOI as long as volume does not influence attachment, as you suggest. I was checking some reference sera with know titre Titred at TCID50 of same virus in some other lab with different cell line, results read at 5th day.

The titers was not even closer to the reference values. Does the reading on 4th or 5th day has something to do with it, or the use of different cell line???? Sir, since tcid50 and pfu are variable from cell line to cell line and day to day of measurement, is it ok to use real time PCR to quantitate viral titre and calculate m.

Hi Prof. I have a phage phix with a titer of 2. The recommended MOI for this phage is 0. I want to titer it up. Can I use a high host concentration e. Based on the volumes I would use the MOI would be somewhere around 0. In my research i need to find effect of MOI, i mean ranges ex. And i got different strains in different moi too ranges from moi In this case should i use Poisson distribution. Actually what is a purpose of Poisson distribution.

Currently we are developing the procedure of antivirals evaluation that is supposed to be common for several groups of researchers. After a discussion we appeared not to be able to come to equivocal opinion and procedure. In my message I would like to kindly ask you to clarify your approach to evaluation of anti-viral potential of chemical compounds. Please excuse me in advance that I ask you to explain obvious things. We study anti-viral properties of various chemical compounds, in particular in cell culture.

Our primary goal is to determine three characteristics for each compound. Third, selectivity index SI , ratio of first to second, the value showing how selectively the given compound kills virus comparing to cells. The minimum purchase order quantity for the product is 1. Pois Moi bracelet in 18kt gold with diamonds. Light in its entirety in a single bracelet. Being a handmade product the above characteristics may be subject to slight variations.

Click here to view the size guide. Pourquoi il vient me parler? Non merci! Il a donc autant peur que sa de moi? Tu sais J'ai honte quand Mais je m'en fiche de sa vie, il peut en faire ce qu'il veux et faire ce qu'il pense je m'en fiche. Et puis, je vois pas pourquoi il aurait honte? Et encore, il m'a fallu un moment! MOI is related to pfu by the following formula:. The moi will be 0. See more in the attached. University of Texas Medical School. Thank you all for the reply.

Sintayehu Fekadu. Hello dear Jaya,. Regarding MOI, you can calculate simply making equal ratio of virus particle to cells to be infected. So according to your virus titre and MOI of 1, you will use 2ul of the virus solution. Thank you. I will. Oliver Thompson. Utrecht University. MOI x total amount of cells. Yara Sadek. Ulm University. How could I calculate the amount of bacteria to be added from thr resuspended bacteria to achieve a certain MOI?

I seeded and want an MOI of

Please excuse me in advance that I ask you to explain obvious things. We study anti-viral properties of various chemical compounds, in particular in cell culture. Our primary goal is to determine three characteristics for each compound. Third, selectivity index SI , ratio of first to second, the value showing how selectively the given compound kills virus comparing to cells.

The higher SI is, the more selective and prospective is the compound. The main contradiction is how to calculate in fact, determine IC To do this, we mark the Y axis as 1, 2, 3, 4, 5, etc.

Therefore, IC50 should decrease the titer twice comparing to control, regardless to the value of the titer in the control no drugs , e. If we follow the opposite logic, we consider the virus titer as absolute, not logarithmic, value.

In this case IC50 should decrease it from 6 to three, or from 4 to 2, and so on. In fact, this will correspond in first case to fold decrease of virus production and in second case — to fold, i. That is why I consider this approach incorrect. In this case the selectivity is based not on properties of a given compound but on different approaches to cells and virus.

I hope then to calculate back the number of cells that were in the dish and thus determine titre….? You could have harvested a large enough volume of cell suspension, obtained a count, and have adjusted the concentration to whatever MOI you want at the time of infection.

In considering multiplicity of infection, is there a volume parameter? It seems to me that a million cells in a culture of a million particles will yield different infection rates in different volumes uL, 1 mL, 10 mL. I asked myself the same question a couple of years ago and tried it out with lentivirus.

But you are correct, there is an unspoken or assumed standardization of volume, which perpetuates the use of MOI in textbooks and official protocols. The suggested incubation for stock preparation also around days. Next post: TWiV Cocaine, colonies, and chickens. Previous post: Derek Lowe on how science gets done. Content on this site is licensed under a Creative Commons Attribution 3. Multiplicity of infection 13 January The answer is no.

Comments on this entry are closed. Cupton 14 January , pm. Alex Ling 15 January , am. Your initial formula for the Poisson distribution looks wrong, btw.

Jahnviyashvi 20 January , pm. Anonymous 20 January , pm. VirVec 23 January , am. Jahnviyashvi 31 January , pm. Nicola Cocacola 8 September , am. Thuyniheje 28 October , pm.

Hovakim 13 December , pm. Thanks in advance. The answer is yes. No matter how you calculate viral titer, the rules of MOI apply. Bio Sunit 6 February , am. Ngunjiri 6 February , pm. Sagar 25 February , am. Francesc Cunyat 9 March , am. Mandeepguru Guru 26 April , am. A phage infects bacteria at a multiplicity of infection moi of 0. This means that. WannaBeGuru 5 July , pm.

Ruiyue Shi. Genemedi Biotechnology Co. Hi, dear Jaya,. MOI multiplicity of infection refers to the number of infected viral particles per cell. For your second question, different cell types have different MOI. For the non-dividing cells, like primary cells with a low infection efficiency, it is better to test a range of MOIs to determine the optimal MOI for infection and gene expression.

Genemedi got a rich experience in lentivirus production and infection, you could find more information about lentivirus infections on this website: www. All Answers Tom Masi. Hi Jaya,. An MOI of 1 is equal number of cells and virus particles. So in your case you would use 2ul of virus for 10, cells. An MOI of 2 would be twice the number of virus particles compared to cells.

It is difficult to answer your second question since you do not mention the cell type you are infecting. Different cell types require different MOIs to achieve a particular transduction efficiency. You should check the literature to see what MOIs people use.

Good Luck! Khashayar Hesamizadeh. Iran Hepatitis Network. Dear Jaya,. MOI is related to pfu by the following formula:. The moi will be 0. See more in the attached. University of Texas Medical School. The minimum purchase order quantity for the product is 1.

Pois Moi bracelet in 18kt gold with diamonds. Light in its entirety in a single bracelet. Being a handmade product the above characteristics may be subject to slight variations. Click here to view the size guide. Add to cart. Add to wishlist.